Elucidation of a masked repeating structure of the O-specific polysaccharide of the halotolerant soil bacteria Azospirillum halopraeferens Au4

• Elena N. Sigida,
• Yuliya P. Fedonenko,
• Alexander S. Shashkov,
• Nikolay P. Arbatsky,
• Evelina L. Zdorovenko,
• Svetlana A. Konnova,
• Vladimir V. Ignatov and
• Yuriy A. Knirel

Beilstein J. Org. Chem. 2016, 12, 636–642, doi:10.3762/bjoc.12.62

• hydrolysis of the lipopolysaccharide isolated by the phenol–water extraction from the halotolerant soil bacteria Azospirillum halopraeferens type strain Au4. The polysaccharide was studied by sugar and methylation analyses, selective cleavages by Smith degradation and solvolysis with trifluoroacetic acid

• residue (~65%), are shown in italics. Keywords: Azospirillum halopraeferens; bacterial polysaccharide structure; lipopolysaccharide; O-specific polysaccharide; Smith degradation; triflic acid solvolysis; Introduction Rhizobacteria of the genus Azospirillum are isolated from a wide variety of

• Azospirillum halopraeferens are isolated from the rhizoplane of kallar grass (Leptochloa fusca L. Kunth), which is widely distributed in tropical and subtropical regions [5] and was successfully introduced as a pioneer plant in salt-contaminated infertile areas [6]. Being halotolerant, A. halopraeferens